Overview
GHK-Cu (glycyl-L-histidyl-L-lysine:copper complex) is a naturally occurring copper peptide with documented involvement in wound healing, tissue remodeling, and skin regeneration. Research indicates activity in gene regulation related to collagen synthesis, antioxidant defense, and anti-inflammatory pathways.
What Is GHK-Cu?
GHK-Cu represents a tripeptide that binds with copper ions to modulate biological processes. The compound demonstrates involvement in wound healing through enhanced collagen and decorin gene expression, activation of tissue remodeling pathways, and regulation of genes associated with antioxidant defense and inflammatory responses.
Reconstitution Instructions
– Volume: Add 3.0 mL sterile or bacteriostatic water to the 50 mg vial
– Final Concentration: 16.67 mg/mL
– Method: Inject water slowly down the vial wall; gently swirl until fully dissolved (avoid vigorous shaking)
– Storage After Reconstitution: Refrigerate at 2–8°C (35.6–46.4°F); use within 30 days
– Measurement: At this concentration, 1 unit on a U-100 insulin syringe equals approximately 167 mcg
Dosage Protocols
Standard/Conservative Protocol (5 days/week)
| Weeks | Dose per Injection | Units (mL) |
|---|---|---|
| 1–4 | 1.0 mg (1000 mcg) | 6 units (0.06 mL) |
| 5–8 | 1.5 mg (1500 mcg) | 9 units (0.09 mL) |
| 9–12+ | 2.0 mg (2000 mcg) | 12 units (0.12 mL) |
Frequency: Once daily, 5 days per week via subcutaneous injection
Alternative Protocol (3x weekly)
| Duration | Dose per Injection | Units (mL) |
|---|---|---|
| Weeks 1–12+ | 2.0 mg (2000 mcg) | 12 units (0.12 mL) |
Frequency: 2 mg three times per week (e.g., Monday/Wednesday/Friday)
Cycle Length
– Standard protocol: 8–12 weeks
– Extended protocol: May continue for 16 weeks based on individual goals
– Rotation strategy: Systematically rotate injection sites to prevent irritation
Storage Instructions
Lyophilized (Powder)
– Temperature: −20°C (−4°F) or lower
– Conditions: Keep in dry, dark environment; maintain desiccant in vial seal
– Duration: Long-term stability with proper storage
Reconstituted Solution
– Temperature: 2–8°C (35.6–46.4°F)
– Duration: Use within 30 days of reconstitution
– Special Note: Bacteriostatic water helps inhibit bacterial growth
– Important: “Avoid repeated freeze–thaw cycles as they degrade peptides over time”
Injection Technique
– Clean vial stopper and injection site with alcohol swabs; allow to air-dry completely
– Pinch skin fold and insert needle at 45–90° angle into subcutaneous tissue
– Do not aspirate for subcutaneous injections
– Inject slowly and steadily
– Withdraw needle at same angle as insertion
– Rotate injection sites (abdomen at least 1 inch from navel, outer thighs, upper arms)
– Use new sterile insulin syringes for each injection
Supplies Needed (8–16 Week Protocol)
For 5 days/week (1.0–2.0 mg/day)
– 8 weeks: ~1 vial
– 12 weeks: ~2 vials
– 16 weeks: ~3 vials
For 3x/week (2 mg each)
– 8 weeks: ~1 vial
– 12 weeks: ~2 vials
– 16 weeks: ~2 vials
Additional Supplies
– U-100 insulin syringes (29–31 gauge): 40–80 per 8–16 week cycle depending on frequency
– Sterile or bacteriostatic water: 1–3 x 10 mL bottles
– Alcohol swabs: 80–160 per 8–16 week cycle
Potential Benefits
– Supports wound healing and tissue repair through collagen synthesis
– Demonstrates positive regulatory effects on genes related to antioxidant enzymes, growth factors, and anti-inflammatory signaling
– Shows activity in nervous system function and cognitive pathways in preclinical models
Side Effects & Safety
– Generally well tolerated
– Most common side effects: mild injection-site reactions (temporary redness, itching) with subcutaneous administration
– No official human dosing guidelines exist; protocols are based on clinical practice and empirical use
– Not FDA-approved; intended for research purposes only
Important Notes
– Document dose, date, and injection site for tracking and consistency
– Clean injection sites properly before administration
– Dispose of syringes in proper sharps containers
– Allow reconstituted vials to reach room temperature before opening to reduce condensation
– Preclinical studies demonstrate activity at very low doses (~0.5 mcg/kg in rodent models), while clinical practice employs milligram-range dosing in humans
Lifestyle Support
– Maintain adequate protein intake for collagen synthesis
– Ensure sufficient hydration and micronutrient status (particularly copper, zinc, vitamin C)
– Prioritize sleep quality and stress management for regenerative processes
– Integrate appropriate skincare or wound-care protocols as needed
Disclaimer
This guide is for educational purposes only and does not constitute medical advice, diagnosis, or treatment. GHK-Cu is not FDA-approved. Always consult a qualified healthcare professional before beginning any research protocol.