Overview
Semax is a synthetic heptapeptide analog of ACTH(4–10), originally developed in Russia for cognitive enhancement and neuroprotective purposes. While intranasal administration is most common in clinical studies, subcutaneous injection offers a practical once-daily alternative for research applications.
Quick Start Summary
– Reconstitution: Add 3.0 mL bacteriostatic water to achieve ~3.33 mg/mL concentration
– Typical range: 300–800 mcg administered once daily
– Measurement: 1 unit on U-100 insulin syringe ≈ 33.3 mcg
– Storage: Keep lyophilized vials at −20 °C; refrigerate reconstituted solution at 2–8 °C for up to 30 days
– Avoid: Freeze-thaw cycles and vigorous shaking
Dosing Protocol
Gradual Titration Schedule (8 weeks)
| Week | Daily Dose | Syringe Units |
|---|---|---|
| 1–2 | 300 mcg | 9 units (0.09 mL) |
| 3–4 | 500 mcg | 15 units (0.15 mL) |
| 5–6 | 600 mcg | 18 units (0.18 mL) |
| 7–8 | 800 mcg | 24 units (0.24 mL) |
Frequency: Once daily via subcutaneous injection
Cycle length: 8 weeks continuous; extensions to 12–16 weeks possible with rest periods (e.g., 6 weeks on, 2 weeks off)
Reconstitution Instructions
1. Draw 3.0 mL bacteriostatic water with sterile syringe
2. Inject slowly down the vial wall to prevent foaming
3. Gently swirl or roll the vial until fully dissolved
4. Label with reconstitution date
5. Refrigerate immediately at 2–8 °C in a light-protected container
Supplies Needed (Example: 8-week protocol)
– Semax vials: 4 × 10mg
– Insulin syringes (U-100): 56 syringes
– Bacteriostatic water: 2 × 10mL bottles
– Alcohol swabs: 2 × 100-count boxes
Storage Guidelines
Lyophilized vials:
– Store at −20 °C (−4 °F) in dry, dark conditions
– Minimize moisture exposure
– Protect from light
Reconstituted solution:
– Refrigerate at 2–8 °C (35.6–46.4 °F)
– Stable for up to 30 days
– Avoid freeze-thaw cycles
– Wrap in foil or store in opaque container
How Semax Works
Semax modulates brain-derived neurotrophic factor (BDNF) expression, enhances cholinergic and dopaminergic neurotransmission, and exhibits neuroprotective properties. Clinical research, primarily from Russia, has explored its use for cognitive support and neurological conditions including mild cognitive impairment and stroke recovery.
Potential Benefits
Research suggests Semax may:
– Support attention and memory consolidation
– Enhance learning in individuals with cognitive deficits
– Provide neuroprotection in models of cerebrovascular injury
– Display favorable safety profiles in month-long human trials
Safety Considerations
– Generally well tolerated
– Intranasal use may cause minor nasal irritation
– Subcutaneous injection may produce mild local reactions (redness, itching)
– No significant cortisol elevation or adverse endocrine effects reported
– Effects are dose-dependent; titration helps identify individual response thresholds
Injection Technique
– Clean vial stopper and injection site with alcohol swabs; allow to dry
– Pinch a 1–2 inch skinfold
– Insert needle at 45–90° angle into subcutaneous tissue
– Do not aspirate before injection
– Inject slowly and steadily
– Withdraw needle at same angle; apply gentle pressure
– Rotate injection sites (abdomen, thighs, upper arms) at least 1–2 inches apart
– Use new sterile syringe for each injection
– Dispose in sharps container
Important Notes
– Document daily dose, injection time, and site rotation for consistency
– Most human data support 4–8 weeks continuous use
– Longer protocols should incorporate rest periods
– Maintain consistent sleep (7–9 hours nightly)
– Engage in regular aerobic exercise and cognitive training
– Follow balanced diet rich in omega-3 fatty acids and antioxidants
– Manage stress through mindfulness or meditation
Disclaimer
This content is for educational and research purposes only. It is not medical advice, diagnosis, or treatment. Always consult qualified healthcare professionals before beginning any research protocol. Not for human consumption.