Overview
5-Amino-1MQ is a small-molecule inhibitor of nicotinamide N-methyltransferase (NNMT) investigated in preclinical research for metabolic effects. By blocking NNMT, the compound preserves cellular NAD⁺ levels and shifts adipose tissue metabolism toward increased energy use and reduced fat accumulation.
Quick Start Summary
– Reconstitution: Add 3.0 mL bacteriostatic water to create ~3.33 mg/mL concentration
– Dosing Range: 150–600 mcg once daily via subcutaneous injection
– Administration: Gradual titration over 8 weeks
– Measurement: At standard concentration, 1 insulin unit ≈ 33.3 mcg
– Storage (Lyophilized): −20 °C (−4 °F) in dark, dry conditions
– Storage (Reconstituted): 2–8 °C (35.6–46.4 °F); avoid freeze-thaw cycles
Dosing Protocol – Standard Gradual Approach
| Week | Daily Dose | Insulin Units | Volume (mL) |
|---|---|---|---|
| 1–2 | 150 mcg | 4.5 units | 0.045 mL |
| 3–4 | 300 mcg | 9.0 units | 0.090 mL |
| 5–8 | 600 mcg | 18 units | 0.18 mL |
Frequency: Once daily subcutaneous injection with consistent timing and rotated injection sites.
Cycle Duration: Minimum 8 weeks; may be extended based on research objectives.
Reconstitution Instructions
1. Draw 3.0 mL of bacteriostatic water using a sterile syringe
2. Inject slowly down the vial wall to minimize foaming
3. Gently swirl or roll until fully dissolved (do not shake vigorously)
4. Label with date and reconstitution details
5. Refrigerate at 2–8 °C in light-protected container
Supplies Needed (8-Week Protocol)
5-Amino-1MQ Vials: Approximately 3 vials for 8-week cycle
Insulin Syringes (U-100): 56 syringes (7 per week for daily injections); consider 30-unit or 50-unit syringes for improved accuracy with small volumes
Bacteriostatic Water: 9 mL total (one 10 mL bottle)
Alcohol Swabs: 112 total (2 per injection day); recommend two 100-count boxes
How It Works
The mechanism involves enzyme inhibition that preserves NAD⁺ availability in cells. Animal research demonstrated that mice receiving 5-Amino-1MQ showed “significant reductions in body weight and adiposity observed in obese mice without changes in food intake.” Additionally, aged mice showed “improved muscle strength and metabolic markers,” suggesting multi-tissue benefits beyond adipose tissue.
Potential Benefits (Preclinical Data)
– Body weight and fat mass reduction in obese animal models
– Enhanced muscle strength and metabolic function in aged models
– Preserved metabolic flexibility without appetite suppression
– Animal studies indicated good tolerance at effective doses
Possible Side Effects
– Generally well-tolerated in preclinical models at therapeutic doses
– Occasional mild injection-site reactions (redness, minor itching)
– No significant adverse effects reported in animal studies
Injection Technique
1. Preparation: Clean vial stopper and injection site with separate alcohol swabs; allow to air dry
2. Technique: Pinch a skinfold; insert needle at 45–90° angle into subcutaneous tissue
3. Administration: Do not aspirate; inject slowly and steadily
4. Site Rotation: Systematically rotate among abdomen, thighs, and upper arms to prevent lipohypertrophy
5. Post-Injection: Apply gentle pressure with gauze if needed; do not rub injection site
Important Practical Notes
– Use fresh sterile syringes for each injection; dispose in sharps container
– Maintain a daily log documenting dose, timing, and injection site
– Allow reconstituted vials to reach room temperature before opening to reduce condensation
– Smaller volumes (≤0.10 mL) are more accurately measured with 30- or 50-unit syringes
– Maintain consistent injection timing for sustained NNMT inhibition
Complementary Lifestyle Strategies
– Combine with balanced, protein-adequate nutrition matched to individual energy needs
– Incorporate resistance training and aerobic activity to reinforce metabolic changes
– Prioritize sleep quality and stress management for optimal recovery
– Monitor body composition changes rather than relying solely on scale weight
– Maintain adequate hydration throughout protocol
Storage Guidelines
Lyophilized Powder:
– Store at −20 °C (−4 °F)
– Keep in dark, dry environment
– Minimize moisture exposure
– Typical shelf life several years when properly stored
Reconstituted Solution:
– Refrigerate at 2–8 °C (35.6–46.4 °F)
– Protect from light exposure
– Avoid repeated freeze-thaw cycles
– Typical usable lifespan 3–4 weeks when refrigerated
Important Disclaimers
This protocol is educational in nature and does not constitute medical advice. All current evidence derives from preclinical research in cells and animal models; no published human clinical trials exist to date. This material is for research purposes only and should not be interpreted as medical guidance for human use.