Overview
Crystagen (Glu-Asp-Pro) is a synthetic tripeptide bioregulator designed to support immune system modulation and cellular regulation. This educational protocol presents guidance for once-daily subcutaneous administration.
Key Information
Peptide Type: Tripeptide bioregulator
Vial Size: 20 mg
Research Application: Immune system support and cellular regulation
Administration Route: Subcutaneous injection
Reconstitution Guide
Standard Approach (3 mL Dilution)
– Reconstitution Volume: 3.0 mL bacteriostatic water
– Final Concentration: 6.67 mg/mL
– Measuring Unit: 1 unit (U-100 insulin syringe) ≈ 66.7 mcg
Reconstitution Steps
1. Draw 3.0 mL bacteriostatic water with sterile syringe
2. Inject slowly down vial wall to avoid foaming
3. Gently swirl/roll until fully dissolved (do not shake)
4. Label vial and refrigerate at 4°C (39.2°F), protected from light
Dosing Protocol
| Week | Daily Dose | Units (U-100) | Volume (mL) |
|---|---|---|---|
| Week 1 | 1000 mcg (1 mg) | 15 units | 0.15 mL |
| Week 2 | 1500 mcg (1.5 mg) | 23 units | 0.23 mL |
| Weeks 3-12 | 2000 mcg (2 mg) | 30 units | 0.30 mL |
Frequency: Once daily via subcutaneous injection
Timing: Consistent daily timing recommended
Cycle Length: 8-12 weeks standard; optional extension to 16 weeks
Storage Instructions
Lyophilized Form:
– Refrigerate at 4°C (39.2°F) or freeze at −20°C (−4°F)
– Store in dry, dark conditions
– Minimize moisture exposure
Reconstituted Solution:
– Refrigerate at 4°C (39.2°F)
– Stable for approximately 30 days
– Discard multi-use bacteriostatic water vials 28 days after opening per USP standards
Supplies Needed (8-16 Week Protocol)
Peptide Vials:
– 8 weeks: ~6 vials
– 12 weeks: ~9 vials
– 16 weeks: ~12 vials
Insulin Syringes (U-100):
– 8 weeks: 56 syringes
– 12 weeks: 84 syringes
– 16 weeks: 112 syringes
Bacteriostatic Water:
– 8 weeks (6 vials): 18 mL → 1×30 mL bottle
– 12 weeks (9 vials): 27 mL → 2×30 mL bottles
– 16 weeks (12 vials): 36 mL → 2-3×30 mL bottles
Alcohol Swabs:
– 8 weeks: 112 swabs (2 per day)
– 12 weeks: 168 swabs
– 16 weeks: 224 swabs
Mechanism of Action
Crystagen operates as a bioregulatory peptide that acts on immune system cells to support cellular regulation and immune function. Research indicates thymic-derived peptides and synthetic analogs can modulate immune responses and support normal cellular activity. Similar immunomodulatory peptides have been studied in clinical settings with daily subcutaneous or intramuscular injection protocols.
Potential Benefits
– May support immune system modulation through bioregulatory peptide signaling
– Related thymic peptides have been researched for immune support in various contexts
– Generally well tolerated in research settings
– Short-course thymic peptide protocols typically range from 5-10 days to several weeks
Side Effects & Considerations
– Occasional mild injection-site reactions (redness, minor discomfort) may occur with subcutaneous administration
– Generally well-tolerated when proper injection technique is followed
– Always monitor injection sites for irritation
Important Notes
– Use new sterile insulin syringes for each injection; dispose in sharps container
– Rotate injection sites systematically (abdomen, thighs, upper arms) to prevent irritation and lipohypertrophy
– Inject slowly; wait seconds before withdrawing needle
– Document daily dose, injection site, and observations
– Follow aseptic technique: wash hands thoroughly and cleanse skin with alcohol before each injection
– Allow lyophilized vials to reach room temperature before opening to reduce condensation
Injection Technique
Site Selection: Choose fatty subcutaneous areas (abdomen, outer thigh, upper arm); avoid irritated or scarred skin
Injection Angle: Insert needle at 45-90° into subcutaneous tissue after pinching approximately 1-inch skinfold
Administration: Do not aspirate for subcutaneous injections; inject slowly and steadily
Post-Injection: Withdraw needle and apply gentle pressure with clean gauze; do not rub site
Site Rotation: Space injections at least 1 inch apart; rotate systematically to prevent local irritation
Needle Safety: Always use new sterile syringe and needle for each injection; never reuse needles
Lifestyle Factors
– Maintain balanced, nutrient-dense diet to support immune and cellular health
– Prioritize 7-9 hours of adequate sleep for optimal recovery
– Manage stress through meditation, exercise, or time management
– Stay hydrated and maintain regular physical activity appropriate to individual capacity
Important Disclaimer
This content is for therapeutic educational purposes only and does not constitute medical advice, diagnosis, or treatment. Crystagen is for research purposes only and not intended for human consumption. Always consult qualified healthcare professionals before beginning any research protocol.