Overview
Pinealon (EDR; tripeptide sequence Glu-Asp-Arg) is investigated for neuroprotective and epigenetic regulatory effects, primarily through in vitro and animal studies with limited human observations.
“Peer-reviewed reports most often describe oral administration in human contexts; subcutaneous human dosing for Pinealon is not established”
Key Information
– Vial Size: 20 mg
– Reconstitution: 3.0 mL bacteriostatic water yields ~6.67 mg/mL concentration
– Insulin Syringe Conversion: At this concentration, 1 unit = approximately 66.7 mcg on a U-100 syringe
– Route: Subcutaneous (research framework only)
– Frequency: Once daily
Dosing Protocol: Gradual Titration
| Week | Daily Dose | Units per Injection | mL per Injection |
|---|---|---|---|
| 1-2 | 200 mcg | 3 units | 0.03 mL |
| 3-4 | 300 mcg | 4.5 units | 0.045 mL |
| 5-6 | 400 mcg | 6 units | 0.06 mL |
| 7-12 | 500 mcg | 7.5 units | 0.075 mL |
Reconstitution Instructions
1. Draw 3.0 mL bacteriostatic water with sterile syringe
2. Inject slowly down vial wall (avoid foaming)
3. Gently swirl/roll until dissolved (do not shake)
4. Label and refrigerate at 2-8°C, protected from light
Storage Guidelines
Lyophilized: Store at −20°C (−4°F) in dry, dark conditions
Reconstituted: Refrigerate at 2-8°C; minimize freeze-thaw cycles
Cycle Information
– Duration: 8-12 weeks (optional extension to 16 weeks)
– Start: 200-300 mcg daily
– Target: 400-500 mcg daily by weeks 5-12
– Timing: Any consistent time; rotate injection sites
Supplies Needed (8-week cycle)
– Peptide: 1 vial (≈19.6 mg total)
– Insulin Syringes (U-100): 56 syringes
– Bacteriostatic Water: 1 × 10 mL bottle
– Alcohol Swabs: 2 × 100-count boxes
Mechanism of Action
Research suggests the peptide may interact with chromatin and regulatory elements, modulating gene expression relevant to neuronal stress responses.
“Animal and cellular studies report improved viability under oxidative stress and cognitive/behavioral signals”
Potential Benefits
– Neuroprotective and antioxidative effects (preclinical data)
– Epigenetic modulation
– Preservation of neuronal structures (spines/synapses)
Side Effects & Considerations
– Limited human safety data for subcutaneous administration
– Possible mild local reactions (redness/itching) at injection sites
– Site rotation essential to prevent lipohypertrophy
Injection Technique
– Clean vial stopper and skin with alcohol swabs
– Pinch skinfold; insert needle at 45-90° angle into subcutaneous tissue
– Aspiration not recommended for routine injections
– Rotate sites (abdomen, thighs, upper arms)
Important Notes
– Use new sterile syringes for each injection
– Dispose needles in sharps container
– Document daily dose and site rotation
– Inject slowly; wait seconds before withdrawing needle
Disclaimer
This protocol is for educational research purposes only and does not constitute medical advice, diagnosis, or treatment. Subcutaneous dosing parameters for Pinealon remain clinically unestablished.